Smt. Mallawabai Valyal Memorial Charitable Dental Hospital & Research Centre's
PANDIT DEENDAYAL UPADHYAY DENTAL COLLEGE
 (Approved by DCI. Affiliated to MUHS Nashik)
19/1, Kegaon, Solapur - 413 225 Maharashtra - India.        
Ph. Nos. : 0217-2500440 / 41.                                 Fax :0217- 2500613

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Histology Stains

Department : Opath

Histological stains are dyes that bind to various tissues and give meaningful diagnostic information of the tissues available.

Types of Stain and Salient features:

  1. Haematoxylin & Eosin stain

    1. most widely used and principal stain in histology.

    2. Haematoxylin stains cell nuclei a purplish blue and eosin stains the extracellular matrix and cytoplasm pink.

  2. Masson Trichome stain:

    1. used for staining collagen, fibrin, muscles and erythrocytes

    2. uses the combination of 3 Stains

      1. weigert's hematoxylin

      2. Biebrich scarlet

      3. Aniline blue

  3. Van Gieson's stain

    1. It is a mixture of picric acid and fuchsin.

    2. It is used for differentiate between collagen and smooth muscle in tumours

    3. The nuclei is stained blue/black. collagen-Red

    4. cytoplasm, muscle, fibrin, RBC stained yellow

  4. Perl's prussian blue stain

    1. It is used to demonstrate the distribution and amount of iron deposits in liver tissue, often in the form of biopsy.

    2. Especially used in bone- marrow and spleen.

    3. Ib stairs thon deposits spleen.

  5. PAS stain (Periodic acid schiff):

    1. It is used to detect glycogen - deposit the liver to detect mucopolysaccharides glycoproteins, glycolipids

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